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KMID : 0350519940470041807
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Journal of Catholic Medical College
1994 Volume.47 No. 4 p.1807 ~ p.1820
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Effects of Insuline and Human Chorionic Gonadotropin on epidermal Growth Factor Deficiency and Oligozoospermia in Streptozotocin Induced Diabetic Mice
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Abstract
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Epidermal growth factor(EGF) which is produced in large amounts in the submandibular glands under the influence of testosterone plays an important role in the spermatogenesis. A number of studies have documented that oligozoospermia is associated
with
diabetes mellitus, but its pathogenesis is not fully understood. Thus, this study was undertaken to determine the effects of insulin and hGG on EGF deficiency and oligozoospermia using diabetic mice.
Ninety six male mice were divided into five groups; In control group(n=24), mice were injected intraperitoneally with normal saline. Insulin-reponsive diabetic mice(DM group, n=24) were produced by intraperitoneal injection of streptozotocin at a
dose
of 50μg/g body weight for vie consecutive days. Treatments with insulin(human NPH insulin, 1 U/0.1ml saline, daily, DI group, n=16), hGG(human chorionic gonadotropin, 0.2U/g body weight, twice a week, DH group n=16) or insulin plus hGG (DIH
group,
n=16) were started 1 week after the first injection of streptozotocin and continued for four weeks.
@ES The results are as follows:
@EN 1. The weights of bodies and testes of DM group, 23.8±1.6g and 89.9±6.3mg, were significantly lower(P<0.01) than those of control group, 33.5±0.9g and 116.7±2.8mg. Those of DI and DIH group was restored to control level.
2. EGF levels in the submandibular glands and plasma of DM group, 63.0±4.4pg/mg tissue and 1.0±0.1ng/ml, were significantly lower(P<0.01). than those of control group, 157.6±0.3pg/mg tissue and 1.7±0.1ng/ml EGF levels in the submandibular
glands in
DI, DH and DIH group and plasma EGF levels in DH and DIH group were completely restored to control level, while plasma EGF level of DI group showed partial recovery.
3. The mean epididymal sperm counts in DM group, 2.3±0.2×10E6/epididymis, was significantly lower(P<0.01) than that of control group, 13.1±0.6×10E6/epididymis. The mean sperm counts of DH and DIH group were completely restored to control
level,
while that of DI group showed partial recovery.
4. The mean plasma testosterone level of DM group, 0.9±0.3ng/ml, was significantly lower (P<0.01) than that of control group, 2.7±0.5ng/ml. The plasma testosterone levels of DI, DH and DIH group were restored to control level.
5. The submandibular glands of DM group showed atrophy of acinar cells and intracytoplasmic granules of the granular convoluted tubule(GCT) cells as compared with those of control. Immunostaining of EGF and EGF receptor(EGFR) in the
submandibular
gland
of the control mice showed specific immunoactivity in the GCT cells, while was completely absent from the acinar cells. The GCT cells of DM group showed decreased EGF immunoactivity and increased EGFR immunoactivity compared with those of the
control.
The seminiferous tubules in DM group showed disorganized, premature sloughing of epithelium and pycnosis of seminiferous epithelial and interstitial cells. Immunostaining of EGF and EGFR in the testis of the control mice showed specific
immunoactivity
of the Sertoli and Leydig cells. The testis of DM group showed decreased EGF immunoactivity of the Sertoli and Leydig cells and decreased EGFR immunoactivity of Leydig cells, while increased of Sertoli cells, which suggests that the EGFR
immunoactitivity of Sertoli cells increased when tubular damage was present.
These results suggest that EGF deficiency pays an important pole, at least in part, in the pathogenesis of oligozoospermia in diabetic mice, which can be reversed by treatment of insulin and hGG.
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KEYWORD
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